SKU: PKSH033972_10μg-ES

Recombinant Human Wnt3a/Wnt-3a, 10 μg

Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes.Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro).

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Product Specification

Basic Information

Brands:Elabscience

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Place of Origin:China

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Reconstitution:Not Applicable

Shipping:This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

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Calculated Molecular Weight:58.5 kDa

Observed Molecular Weight:66-73 kDa

Purity: > 95 % as determined by reducing SDS-PAGE.

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Storage:Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

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Calculated Molecular Weight:58.5 kDa

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Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method.

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Application:Cell culture

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Bio-activity:Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.The ED50 for this effect is 71.45 ng/ml.

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Formulation:Supplied as a 0.2 μm filtered solution of 25mM Tris-HCl, 500mM NaCl, pH8.2.

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