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SKU: PKSH032966_50μg-ES
Recombinant Human WDYHV1/NTAQ1 Protein (GST Tag), 50 μg
Human protein N-terminal glutamine amidohydrolase (WDYHV1) is an enzyme that in humans is encoded by the WDYHV1 gene, belongs to the NTAQ1 family.WDYHV1 mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, which is an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. However,it does not act on substrates with internal or C-terminal glutamine andnon-glutamine residues in any position. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.
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Product Specification
Basic Information
Brands:Elabscience
Model Number:
Place of Origin:China
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Sterile Status:
Reconstitution:Not Applicable
Shipping:This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
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Calculated Molecular Weight:49.8 kDa
Observed Molecular Weight:45-50 kDa
Purity: > 95 % as determined by reducing SDS-PAGE.
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Storage:Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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Calculated Molecular Weight:49.8 kDa
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Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
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Research Areas:Cell biology
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Bio-activity:Not validated for activity
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Formulation:Supplied as a 0.2 μm filtered solution of PBS,100mM GSH,1% TritonX-100,15% Glycerol,pH7.4.
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