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SKU: PKSH033164_100μg-ES
Recombinant Human TNF-alpha/TNFA Protein (His Tag), 100 μg
Tumor Necrosis Factor-α (TNF-α) is secreted by macrophages; monocytes; neutrophils; T-cells; and NK-cells following stimulation by bacterial LPS. Cells expressing CD4 secrete TNF-α while cells that express CD8 secrete little or no TNF-α. Synthesis of TNF-α can be induced by many different stimuli including interferons; IL2; and GM-CSF. The clinical use of the potent anti-tumor activity of TNF-α has been limited by the proinflammatory side effects such as fever; dose-limiting hypotension; hepatotoxicity; intravascular thrombosis; and hemorrhage. Designing clinically applicable TNF-α mutants with low systemic toxicity has been of intense pharmacological interest. Human TNF-α that binds to murine TNF-R55 but not murine TNF-R7; exhibits retained anti-tumor activity and reduced systemic toxicity in mice compared with murine TNF-α; which binds to both murine TNF receptors. Based on these results; many TNF-α mutants that selectively bind to TNF-R55 have been designed. These mutants displayed cytotoxic activities on tumor cell lines in vitro and have exhibited lower systemic toxicity in vivo. Recombinant Human TNF-α High Active Mutant differs from the wild-type by amino acid subsitution of amino acids 1-7 with Arg8; Lys9; Arg10 and Phe157. This mutant form has been shown to have increased activity with less inflammatory side effects in vivo.
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Product Specification
Basic Information
Brands:Elabscience
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Place of Origin:China
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Reconstitution:Please refer to the printed manual for detailed information.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
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Calculated Molecular Weight:18.3 kDa
Observed Molecular Weight:17 kDa
Purity: > 97 % as determined by reducing SDS-PAGE.
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Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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Calculated Molecular Weight:18.3 kDa
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Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.
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Application:Cell culture
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Research Areas:Signal Transduction;Cardiovascular;Cancer;immunology;metabolism;
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Bio-activity:Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED50 for this effect is < 0.1 ng/mL. The specific activity of recombinant human TNF alpha is approximately ≥ 1 x 107 IU/mg.
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Formulation:Lyophilized from sterile PBS,pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
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