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SKU: PKSH033503_10μg-ES
Recombinant Human FGF-2/FGF basic/FGFb Protein, 10 μg
Fibroblast growth factors (FGF) are a family of heparin-binding secreted proteins that stimulate cell proliferation and differentiation in a wide variety of tissues. FGFs play important roles in diverse biological functions both in vivo and in vitro; including mitogenesis; cellular migration; differentiation; angiogenesis; and wound healing. Human embryonic stem cell (hESC) cultures require FGF basic (also known as FGF-2 or bFGF) in cell culture media to remain in an undifferentiated and pluripotent state. Thermostable FGF basic was engineered for enhanced stability in culture media; without modification of its biological function. FGF basic is a required component of stem cell culture media for maintaining cells in an undifferentiated state. Because FGF basic is unstable; daily media changes are needed. The thermostable FGF basic that supports a 2-day media change schedule; so no media changes are required over a weekend. This thermostable FGF basic was more stable than FGF basic in biochemical studies; and maintained cell growth; pluripotency and differentiation potential with a 2-day feeding schedule.
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Product Specification
Basic Information
Brands:Elabscience
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Place of Origin:China
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Sterile Status:
Reconstitution:Not Applicable
Shipping:This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
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Calculated Molecular Weight:17.2 kDa
Observed Molecular Weight:17 kDa
Purity: > 95 % as determined by reducing SDS-PAGE.
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Storage:Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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Calculated Molecular Weight:17.2 kDa
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Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
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Research Areas:Cardiovascular,Signal Transduction,Neuroscience,Stem Cells,Cancer,Developmental Biology
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Bio-activity:Not validated for activity
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Formulation:Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM NaCl, pH 7.5.
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