Mouse ST2 ELISA Kit, 96T

Brands:Elabscience

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Place of Origin:China

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Shipping:Ice bag

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Sensitivity:0.19 ng/mL

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Shelf Life:12 months

Storage:2-8℃/-20℃

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Detection Type:Colormetric

Detection Range: 0.31-20 ng/mL

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Test Principle:This?ELISA?kit?uses?the?Sandwich-ELISA?principle.?The?micro?ELISA?plate?provided?in?this?kit?has?been?pre-coated?with?an?antibody?specific?to?Mouse?ST2.?Standards?or?samples?are?added?to?the?micro?ELISA?plate?wells?and?combined?with?the?specific?antibody.?Then?a?biotinylated?detection?antibody?specific?for?Mouse?ST2?and?Avidin-Horseradish?Peroxidase?(HRP)?conjugate?are?added?successively?to?each?micro?plate?well?and?incubated.?Free?components?are?washed?away.?The?substrate?solution?is?added?to?each?well.?Only?those?wells?that?contain?Mouse?ST2,?biotinylated?detection?antibody?and?Avidin-HRP?conjugate?will?appear?blue?in?color.?The?enzyme-substrate?reaction?is?terminated?by?the?addition?of?stop?solution?and?the?color?turns?yellow.?The?optical?density?(OD)?is?measured?spectrophotometrically?at?a?wavelength?of?450?nm?±?2?nm.?The?OD?value?is?proportional?to?the?concentration?of?Mouse?ST2.?You?can?calculate?the?concentration?of?Mouse?ST2?in?the?samples?by?comparing?the?OD?of?the?samples?to?the?standard?curve.

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Assay Time:3 h 30 min

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