T5 核酸外切酶，5000 U

Brands:Beyotime

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Place of Origin:中國

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Shipping:藍冰/乾冰

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Component:T5 核酸外切酶 (10U/µl)、10X 反應緩衝液

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Shelf Life:36個月

Storage:-20℃

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Test Principle:Beyotime 的 T5 核酸外切酶是一種特定的核酸外切酶，可沿著 5' 到 3' 方向降解雙股 DNA (dsDNA) 和單股 DNA (ssDNA)。它能夠從 5' 末端或線性或環狀 dsDNA 的間隙和缺口處啟動核苷酸去除。但是，它不會降解超螺旋 dsDNA。 T5 核酸外切酶也具有 ssDNA 核酸內切酶活性，可透過將反應混合物中的 Mg2+ 濃度降低至 1mM 以下來抑制此活性。 T5 核酸外切酶在 Gibson 組裝中被廣泛使用。

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