T4 核酸內切酶 V (T4 PDG)，20 KU

Brands:Beyotime

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Place of Origin:中國

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Shipping:乾冰

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Component:T4 核酸內切酶 V（10U/µl）、10X T4 核酸內切酶 V 緩衝液

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Purity:≥ 95%

Shelf Life:24個月

Storage:-20℃

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Test Principle:Beyotime T4 核酸內切酶 V，也稱為 T4 嘧啶二聚體糖基化酶 (T4 PDG)，是一種源自 T4 噬菌體的重組酶，是一種具有 DNA N-糖基化酶和 AP 裂解酶活性的 DNA 損傷修復酶。其 N-糖基化酶活性可辨識紫外線照射引起的順式-順式-環丁烷嘧啶二聚體 (T^T、T^C、C^C) 並裂解 5'-糖苷鍵，產生無嘌呤/無嘧啶 (AP) 位點。然後，其 AP 裂解酶活性透過 β 消除去除 AP 位點，產生具有 3'-α,β-不飽和醛和 5'-磷酸末端的一個核苷酸 DNA 間隙。

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