SARS-CoV-2 刺突蛋白 IgM ELISA 試劑盒，96T

Brands:Elabscience

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Place of Origin:中國

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Shipping:冰袋

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Shelf Life:6個月

Storage:2-8℃

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Detection Type:比色法

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Test Principle:此ELISA試劑盒採用間接ELISA法定性檢測樣本中的SARS-CoV-2 Spike蛋白IgM。本試劑盒提供的微量ELISA板預先包覆有純化的SARS-CoV-2 Spike蛋白（PKSR030489）抗原，將樣本加入孔中後，樣本中的SARS-CoV-2 Spike蛋白IgM將與預先包覆的SARS-CoV-2 Spike蛋白抗原結合。徹底洗滌後，加入辣根過氧化物酶 (HRP) 偶聯的鼠抗人 IgM，形成抗原-抗體-HRP 偶聯的二抗體複合物。洗去遊離成分，然後將底物溶液加入每個孔中。只有含有 SARS-CoV-2 刺突蛋白 IgM 和 HRP 結合抗人 IgM 的孔才會呈現藍色。透過添加終止液來終止酵素-底物反應，並且顏色變成黃色。採用分光光度法在 450 ± 2 nm 的波長下測量光密度 (OD)。與CUT OFF值進行比較，判斷檢測樣本中是否有SARS-CoV-2 Spike Protein IgM。

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Assay Time:1 小時 30 分鐘

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