SARS-CoV-2 中和抗體 ELISA 試劑盒，96T

Brands:Elabscience

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Place of Origin:中國

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Shipping:冰袋

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Sensitivity:9.38 奈克/毫升

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Shelf Life:6個月

Storage:2-8℃

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Detection Type:比色法

Detection Range: 15.63-500 奈克/毫升

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Test Principle:此ELISA試劑盒採用競爭性ELISA方法定性檢測樣本中的抗SARS-CoV-2中和抗體。本試劑盒提供的微量ELISA板預先包覆有重組人ACE2。反應過程中，預處理樣本或對照中的SARS-CoV-2中和抗體與固相載體上一定量的人類ACE2競爭辣根過氧化物酶（HRP）偶聯的重組SARS-CoV-2 RBD片段（HRP-RBD）上的位點。 37℃孵育後，未結合的HRP-RBD以及與非中和抗體結合的任何HRP-RBD將被捕獲在板上並最終形成ACE2-RBD-HRP複合物，而循環中和抗體HRP-RBD複合物仍留在上清液中並在洗滌過程中被移除。然後將 TMB 底物溶液加入每個孔中。透過添加終止液來終止酵素-底物反應，並在 450 nm ± 2 nm 的波長下以分光光度法測量顏色變化。透過抑制率對比判斷檢測樣本中是否有SARS-CoV-2中和抗體。

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Assay Time:1 小時 30 分鐘

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