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Product Specification
Brands:Elabscience
Model Number:
Place of Origin:中國
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Reconstitution:請參閱印刷手冊以取得詳細資訊。
Shipping:本產品以凍乾粉形式提供,並附冰袋運送。
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Calculated Molecular Weight:39.3 kDa
Observed Molecular Weight:50-55kDa
Purity: 以還原 SDS-PAGE 測定,結果為 > 98%。
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Storage:一般來說,冷凍乾燥蛋白在-20至-80℃保存可穩定保存長達12個月。重建後的蛋白溶液可在4-8℃保存2-7天。重建樣品的等分試樣在 < -20℃ 穩定保存 3 個月。
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Calculated Molecular Weight:39.3 kDa
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Endotoxin: 經 LAL 方法測定,每 μg 蛋白質含量 < 1.0 EU。
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Application:細胞培養
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Bio-activity:1. 透過其抑制D10.G4中IL1β依賴性增殖的能力來測量。 1 小鼠輔助T細胞。在 40 pg/mL rhIL1β 存在下,此效果的 ED50 通常為 5.5 μg/mL。 2. 透過測量在 500pg/mL IL1b 存在下其抑制人類自然殺手淋巴瘤 NK-92 細胞分泌幹擾素γ的能力。這種效果的 EC50 通常為 20-80ng/mL。
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Formulation:由無菌 PBS 冷凍乾燥而成,pH 值為 7.4
通常在冷凍乾燥前會添加5%~8%的海藻糖、甘露醇和0.01%的吐溫80作為保護劑。
請參閱印刷手冊中的具體緩衝液資訊。
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