重組人 CD47 蛋白（Fc 標籤），1 毫克

Brands:Elabscience

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Place of Origin:中國

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Reconstitution:請參閱印刷手冊以取得詳細資訊。

Shipping:本產品以凍乾粉形式提供，並附冰袋運送。

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Calculated Molecular Weight:40.7 kDa

Observed Molecular Weight:48-60kDa

Purity: 以還原 SDS-PAGE 測定，結果 > 95%。

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Storage:一般來說，冷凍乾燥蛋白在-20至-80℃保存可穩定保存長達12個月。重建後的蛋白溶液可在4-8℃保存2-7天。重建樣品的等分試樣在 < -20℃ 穩定保存 3 個月。

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Calculated Molecular Weight:40.7 kDa

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Endotoxin: 經 LAL 方法測定，每 μg 蛋白質含量 < 1.0 EU。

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Application:酵素連結免疫吸附試驗

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Bio-activity:1. 固定化的人SIRPA-His以10 μg/ml（100 μl/孔）可結合人類CD47-Fc，人類CD47-Fc的EC50為10.1-23.5 ng/ml。 2. 固定化鼠SIRPA-His以10 μg/ml(100 μl/孔)可結合人類CD47-Fc，人類CD47-Fc的EC50為0.05-0.13 μg/ml。 3. 固定化的人SIRPG-His以10 μg/ml(100 μl/孔)可以與人CD47-Fc結合，人CD47-Fc的EC50為0.58-1。 34 μg/毫升。

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Formulation:由無菌 PBS 冷凍乾燥而成，pH 值為 7.4
通常在冷凍乾燥前會添加5%～8%的海藻糖、甘露醇和0.01%的吐溫80作為保護劑。
請參閱印刷手冊中的具體緩衝液資訊。

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