QuicKey Pro 小鼠 T（睾酮）ELISA 試劑盒，24T

Brands:Elabscience

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Place of Origin:中國

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Shipping:冰袋

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Sensitivity:0.14 奈克/毫升

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Precision:內部 CV 和外部 CV 均 < 10%。

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Shelf Life:6個月

Storage:2-8℃

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Application:本ELISA試劑盒適用於體外定量測定小鼠血清、血漿中T濃度。

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Detection Type:比色法

Detection Range: 0.31-20 奈克/毫升

Research Areas:訊號傳導、訊號傳導、表觀遺傳學和核子訊號傳導、發育生物學

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Test Principle:此 ELISA 試劑盒採用競爭性 ELISA 原理。本試劑盒提供的微量 ELISA 板已預先包覆小鼠 T。將樣本（或標準品）和針對小鼠 T 的辣根過氧化物酶 (HRP) 偶聯抗體加入微量 ELISA 板孔中。樣本（或標準）中的小鼠 T 與固相載體上的固定量的 T 競爭 HRP 連接的針對 T 的檢測抗體上的位點。過量的結合物和未結合的樣品或標準會從平板上洗掉。將底物溶液加入每個孔中。透過添加終止液來終止酵素-底物反應，並且顏色變成黃色。採用分光光度法在450±2nm波長下測量光密度(OD)。然後透過將樣品的 OD 與標準曲線進行比較來確定樣品中 Mouse T 的濃度。

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Assay Time:1 小時 30 分鐘

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