QuicKey Pro 人類 CRP（C 反應蛋白）ELISA 試劑盒，96T

Brands:Elabscience

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Place of Origin:中國

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Sensitivity:52.1 皮克/毫升

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Precision:內部 CV 和外部 CV 均 < 10%。

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Shelf Life:6個月

Storage:2-8℃

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Application:本ELISA試劑盒適用於體外定量測定血清、血漿中人類CRP的濃度。

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Detection Type:比色法

Detection Range: 125-8000 皮克/毫升

Research Areas:癌症、心血管、表觀遺傳學和核子訊號、代謝、免疫學

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Test Principle:此 ELISA 試劑盒採用夾心 ELISA 原理。本試劑盒提供的微型 ELISA 板已預先包覆針對人類 CRP 的特異性抗體。將樣本（或標準）和針對人類 CRP 的辣根過氧化物酶 (HRP) 連接抗體添加到微量 ELISA 板孔中。樣本（或標準品）中的人類CRP與針對人類CRP特異性的包被抗體和HRP標記的檢測抗體結合，過量的結合物和未結合的樣本或標準品從板中洗掉。將底物溶液加入每個孔中。透過添加終止液來終止酵素-底物反應，並且顏色變成黃色。採用分光光度法在450±2nm波長下測量光密度(OD)。 OD值與人CRP的濃度成正比。然後透過將樣本的 OD 與標準曲線進行比較來確定樣本中人類 CRP 的濃度。

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Assay Time:1 小時 30 分鐘

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