快速鈍化套件，20 T

Brands:Beyotime

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Place of Origin:中國

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Shipping:藍冰/乾冰

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Component:鈍化酶混合物、10X 鈍化緩衝液、dNTP 混合物 (1mM)

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Shelf Life:12個月

Storage:-20℃

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Test Principle:Beyotime 的 Quick Blunting Kit 可以將 DNA 片段的 5' 和 3' 懸垂部分快速轉換為平端。兩種懸垂部分的轉換可透過試劑盒提供的 Blunting Enzyme Mix 中所含的 T4 DNA 聚合酶 (D7052) 的 3'→5' DNA 外切酶和 5'→3' DNA 聚合酶活性同時完成。鈍化酶混合物還含有 T4 多核苷酸激酶 (D7098)，它可以磷酸化 5' 端並透過其 3'-脫磷酸化酶活性催化 3'-磷酸轉化為 3'-羥基。

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