一步法TUNEL細胞凋亡檢測試劑盒（綠色螢光），50T

Brands:Beyotime

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Shipping:乾冰

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Component:TdT 酶、螢光素-dUTP 標記溶液、TdT 稀釋緩衝液

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Shelf Life:-20℃保存1年

Storage:-20 ℃

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Em:570奈米

Ex:550奈米

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Test Principle:細胞 DNA 碎片是細胞凋亡的標誌。在正在發生細胞凋亡的細胞中，細胞內切酶被激活，將核小體之間的基因組 DNA 切割成 1-2bp 的 DNA 片段。一步法TUNEL 細胞凋亡檢測試劑盒以酵素末端脫氧核苷酸轉移酶（TdT）催化螢光修飾的dUTP（螢光素-dUTP）摻入片段化DNA的遊離3'-OH末端。這種 TdT 介導的 dUTP 缺口末端標記縮寫為 TUNEL。

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Assay Time:1-2小時

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Detection Instrument :螢光顯微鏡；流式細胞儀

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