小鼠 ST2 ELISA 試劑盒，24T

Brands:Elabscience

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Place of Origin:中國

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Shipping:冰袋

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Sensitivity:0.19 奈克/毫升

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Shelf Life:12個月

Storage:2-8℃/-20℃

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Detection Type:比色法

Detection Range: 0.31-20 奈克/毫升

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Test Principle:本 ELISA 試劑盒採用夾心 ELISA 原理。本試劑盒提供的微量 ELISA 板已預先包覆針對小鼠 ST2 的特異性抗體。將標準品或樣本加入微量 ELISA 板孔中並與特異性抗體結合。然後，將針對小鼠 ST2 的特異性生物素化檢測抗體和親和素-辣根過氧化物酶 (HRP) 結合物依序加入每個微量板孔中並孵育。洗去遊離組分。將底物溶液加入每個孔中。只有含有小鼠 ST2、生物素化檢測抗體和 Avidin-HRP 結合物的孔才會呈現藍色。透過添加終止液來終止酵素-底物反應，顏色變為黃色。在 450 nm ± 2 nm 的波長處以分光光度法測量光密度 (OD)。 OD 值與小鼠 ST2 的濃度成比例。您可以將樣本的 OD 與標準曲線進行比較來計算樣本中小鼠 ST2 的濃度。

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Assay Time:3 小時 30 分鐘

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