微型樣本小鼠 NGF（神經生長因子）ELISA 試劑盒，48T

Brands:Elabscience

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Place of Origin:中國

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Shipping:冰袋

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Sensitivity:9.65 皮克/毫升

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Precision:內部 CV 和外部 CV 均 < 10%。

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Shelf Life:12個月

Storage:2-8℃/-20℃

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Application:此ELISA試劑盒適用於體外定量測定血清、血漿和其他生物體液中小鼠NGF的濃度。

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Detection Type:比色法

Detection Range: 31.25-2000 皮克/毫升

Research Areas:癌症，神經科學

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Test Principle:此 ELISA 試劑盒採用夾心 ELISA 原理。本試劑盒提供的微型 ELISA 板已預先包覆針對小鼠 NGF 的特異性抗體。將標準品或樣本加入微量ELISA板孔中，並與特異性抗體結合。然後將針對小鼠NGF的生物素化檢測抗體和親和素-辣根過氧化物酶（HRP）結合物依序加入每個微孔板孔中並孵育。自由組分被沖走。將底物溶液加入每個孔中。只有含有小鼠 NGF、生物素化檢測抗體和 Avidin-HRP 結合物的孔才會呈現藍色。透過添加終止液來終止酵素-底物反應，並且顏色變成黃色。採用分光光度法在 450 nm ± 2 nm 的波長下測量光密度 (OD)。 OD值與小鼠NGF的濃度成比例。您可以將樣本的 OD 與標準曲線進行比較來計算樣本中小鼠 NGF 的濃度。

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Assay Time:3 小時 30 分鐘

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