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庫存單位: E-EL-H1015_96T-ES
人類 OXA（Orexin A）ELISA 試劑盒，96T
此 ELISA 試劑盒採用競爭性 ELISA 原理。本試劑盒提供的微型 ELISA 板已預先包覆人類 OXA。反應過程中，樣本或標準品中的人類OXA與固相載體上一定量的人OXA競爭針對人類OXA的生物素化檢測抗體上的位點。洗去多餘的結合物和未結合的樣品或標準品，並將親和素-辣根過氧化物酶 (HRP) 結合物添加到每個微孔板孔中並孵育。然後將 TMB 底物溶液加入每個孔中。透過加入終止液來終止酵素-底物反應，顏色由藍色變為黃色。採用分光光度法在 450 nm ± 2 nm 的波長下測量光密度 (OD)。透過將樣本的 OD 與標準曲線進行比較，可以計算出測試樣本中人類 OXA 的濃度。
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Product Specification
Basic Information
Brands:Elabscience
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Place of Origin:China
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Sensitivity:37.5 pg/mL
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Shelf Life:12 months
Storage:2-8℃/-20℃
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Detection Type:Colormetric
Detection Range: 62.5-4000 pg/mL
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Test Principle:This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human OXA. During the reaction, Human OXA in the sample or standard competes with a fixed amount of Human OXA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human OXA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human OXA in tested samples can be calculated by comparing the OD of the samples to the standard curve.
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Assay Time:2 h 30 min
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