彗星檢測試劑盒，100 T

Brands:Beyotime

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Place of Origin:中國

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Shipping:RT/藍冰/乾冰

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Component:裂解緩衝液、DMSO、正常熔點瓊脂糖、低熔點瓊脂糖、碘化丙啶溶液

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Shelf Life:12個月

Storage:4℃

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Em:617奈米

Ex:535奈米

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Test Principle:低熔點瓊脂糖的特徵是在37℃時仍保持液態，有助於維持細胞的原始狀態，而不會造成額外的DNA損傷。因此，細胞可以嵌入玻璃載玻片上的低熔點瓊脂糖凝膠中。細胞裂解後，正常細胞中的大分子DNA在電泳過程中的遷移速度基本上相同，且有非常輕微的拖尾。然而，DNA單股或雙股斷裂會產生不同大小的DNA片段，這些片段在電泳過程中以不同的速率遷移。 DNA損傷越嚴重，DNA片段越小，遷移速度越快，彗星狀拖尾越長。因此，可以透過測量彗星尾部的光密度或長度來量化單一細胞的 DNA 損傷程度。

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Detection Instrument :螢光顯微鏡

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