顯色法鱟試劑盒內毒素檢測試劑盒，160 T

Brands:Beyotime

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Place of Origin:中國

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Shipping:藍冰/乾冰

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Component:內毒素標準品（20EU）、檢測試劑、檢測試劑製備緩衝液、顯色劑、緩衝液A、緩衝液B、緩衝液C、無內毒素水

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Sensitivity:0.1-1.0EU/毫升或0.01-0.10EU/毫升

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Shelf Life:24個月

Storage:4℃

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Detection Type:比色法

Detection Range: 0.1-1.0EU/毫升或0.01-0.10EU/毫升

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Test Principle:冷凍乾燥變形細胞裂解物 (LAL) 是由三齒鱟的變形細胞製成的。它含有因子C、因子B、凝固酶和凝固素原。在適當的條件下，內毒素活化C因子，進而進行一系列酶促反應，活化凝固素原形成凝固酶，凝固酶能裂解本試劑盒中的髮色原，生成黃色的對硝基苯胺（pNA，λmax=405nm）。產生的pNA的量與一定時期內的內毒素濃度呈正相關。同時，pNA可以與偶氮試劑反應，生成玫瑰紅色的終產物(λmax=545nm)，利用此終產物可以測定樣品的內毒素濃度，同時可以避免樣品顏色對405nm處吸光度的干擾。

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Result Type:定量

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Assay Time:16 分鐘

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Detection Instrument :分光光度計或平板讀數儀

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