鈣黃綠素/PI 細胞活力/細胞毒性檢測試劑盒，100T

Brands:Beyotime

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Shipping:乾冰

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Component:鈣黃綠素 AM (1000X)、檢測緩衝液、PI (1000X)

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Shelf Life:-20℃保存1年

Storage:-20 ℃

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Em:514 奈米；617 奈米

Ex:494 奈米；535 奈米

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Test Principle:Calcein AM 是一種非螢光的疏水性化合物，可輕易滲透完整的活細胞。細胞內的酯酶水解 Calcein AM 產生鈣​​黃綠素，它是一種親水性強螢光化合物，可以很好地保留在細胞質中，並用強綠色螢光染色活細胞。死細胞不能用螢光鈣黃綠素標記，因為死細胞沒有或具有極低的酯酶活性。然而，PI 是一種不透細胞膜的染料，只能染色細胞膜完整性受損的死細胞，當與死細胞核中的 DNA 結合時會發出明亮的紅色螢光。因此，Calcein AM可與PI合併使用，同時檢測細胞活力和細胞毒性。

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Result Type:定量

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Assay Time:3 分鐘

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Detection Instrument :流式細胞儀、螢光顯微鏡或螢光微孔板讀數儀

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