生物素定量測定 ELISA 試劑盒，24T

Brands:Elabscience

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Place of Origin:中國

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Shipping:冰袋

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Sensitivity:0.19 奈克/毫升

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Precision:內部 CV 和外部 CV 均 < 10%。

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Shelf Life:12個月

Storage:2-8℃

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Detection Type:比色法

Detection Range: 0.31-20 奈克/毫升

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Test Principle:此 ELISA 試劑盒採用競爭性 ELISA 原理。本試劑盒提供的微型 ELISA 板已預先以生物素包覆。在反應過程中，樣品或標準中的生物素與固相載體上一定量的生物素競爭Avidin-HRP上對生物素特異性的位點。過量的 Avidin-HRP 和未結合的樣品或標準品會從板上洗掉。然後將 TMB 底物溶液加入每個孔中。透過添加終止液來終止酵素-底物反應，並在450±2nm的波長下用分光光度法測量顏色變化。然後透過將樣品的 OD 與標準曲線進行比較來確定樣品中生物素的濃度。

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Assay Time:1 小時 30 分鐘

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