BeyoClick™ EU RNA 合成試劑盒（含 Alexa Fluor 488，50-500T）

Brands:Beyotime

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Shipping:乾冰

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Component:EU（100mM）、疊氮化物 488、點擊反應緩衝液、CuSO4、點擊添加劑、Hoechst 33342（1000X）

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Shelf Life:-20℃保存1年

Storage:-20 ℃

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Em:519奈米

Ex:495奈米

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Test Principle:EU（5-乙炔基尿苷）是一種新型尿苷類似物，可以取代尿苷並摻入新合成的RNA中。檢測基於EU上的炔烴基團與螢光探針標記的小疊氮化物分子（例如疊氮化物 Alexa Fluor 488、疊氮化物 Alexa Fluor 555、疊氮化物 Alexa Fluor 594 和疊氮化物 Alexa Fluor 647）之間的銅催化點擊反應。透過這種快速而特異的點擊反應，新合成的RNA被螢光標記，並可使用適當的螢光檢測儀器進行檢測。

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Detection Instrument :流式細胞儀、螢光顯微鏡、多功能讀板機或高內涵篩選儀

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